著書・論文(1998 年以降)

著書

鈴木史朗・肥塚崇男・明石智義  芳香族化合物(基礎から学ぶ 植物代謝生化学,第 I 部 植物代謝産物の分類と生合成経路,水谷正治・士反伸和・杉山暁史 編)羊土社(2018年12月)

 

綾部真一,明石智義,青木俊夫, 内山寛 イソフラボノイドの生合成(植物色素 フラボノイド,第4章 フラボノイド研究の実際,武田幸作・齋藤規夫・岩科司 編)文一総合出版(2013年6月)

 

Ayabe, S., Uchiyama, S., Aoki, T. and Akashi, T. (2010) Plant Phenolics: Phenylpropanoids, In Comprehensive Natural Products II: Chemistry and Biology, Volume 1: Natural Products Structural Diversity-I Secondary Metabolites: Organization and Biosynthesis (Eds, Lew Mander and Hung-Wen (Ben) Liu), Pages 929-976, Elsevier. [Abstract]

 

綾部真一,内山寛,青木俊夫,明石智義 種子のフェニルプロパノイド生合成(種子の科学とバイオテクノロジー 第3章 種子成分の生合成と生化学,種子生理生化学研究会 編)学会出版センター(2009年4月)

 

Aoki, T. (2008) Development of New Bioremediation Systems of Acid Sulfate Soil for Agriculture and Forestry (担当ページ:41-44, 97-100, 105-108)Shoukadoh Book Sellers.

 

綾部真一・明石智義 フラボノイド,イソフラボノイド(天然物化学(植物編)第4章 二次代謝産物の生合成,山村・長谷川 編)アイピーシー出版部(2006年11月)

 

青木俊夫 ミヤコグサの培養系(改訂3版 モデル植物の実験プロトコール,島本功・岡田清孝・田畑哲之 監修)秀潤社(2005年5月)

 

Ayabe, S., Akashi, T., Aoki, T. (2002) Cloning of cDNAs Encoding P450s in the Flavonoid/Isoflavonoid Pathway from Elicited Leguminous Cell Cultures, In Methods in Enzymology, Cytochrome P450, Part C (Eds, Johnson, E. F., Waterman, M. R.), pp. 360-369, Academic Press, San Diego.

論文

 

[2017] 

  • 明石智義,青木俊夫(2017)触媒活性をもとに植物の酵素遺伝子を捉える.生物工学会誌,95(3): 142. [Original]
  • Kai Uchida, Tomoyoshi Akashi and Toshio Aoki (2017) The Missing Link in Leguminous Pterocarpan Biosynthesis is a Dirigent Domain-Containing Protein with Isoflavanol Dehydratase Activity. Plant and Cell Physiology, 58(2): 398-408. [Pubmed]

 

[2016]

  • Keisuke Yoneyama, Tomoyoshi Akashi and Toshio Aoki (2016) Molecular characterization of soybean pterocarpan 2-dimethylallyltransferase in glyceollin biosynthesis: Local gene and whole-genome duplications of prenyltransferase genes led to the structural diversity of soybean prenylated isoflavonoids. Plant and Cell Physiology, 57(12): 2497-2509. [Pubmed]
  • Yuki Tsukagoshi, Kiyoshi Ohyama, Hikaru Seki, Tomoyoshi Akashi, Toshiya Muranaka, Hideyuki Suzuki and Yoshinori Fujimoto (2016) Functional characterization of CYP71D443, a cytochrome P450 catalyzing C-22 hydroxylation in the 20-hydroxyecdysone biosynthesis of Ajuga hairy roots. Phytochemistry, 127: 23-28. [Pubmed]

 

[2015]

  • Toshiyuki Waki, DongChan Yoo, Naoto Fujino, Ryo Mameda, Konstantin Denessiouk, Satoshi Yamashita, Reiko Motohashi, Tomoyoshi Akashi, Toshio Aoki, Shin-ichi Ayabe, Seiji Takahashi, Toru Nakayama (2015) Identification of protein-protein interactions of isoflavonoid biosynthetic enzymes with 2-hydroxyisoflavanone synthase in soybean (Glycine max (L.) Merr.). Biochemical and Biophysical Research Communications, 469(3): 546-551. [ScienceDirect]
  • Kai Uchida, Tomoyoshi Akashi and Toshio Aoki (2015) Functional expression of cytochrome P450 in Escherichia coli: An approach to functional analysis of uncharacterized enzymes for flavonoid biosynthesis. Plant Biotechnology, 66(11): 205-213. [Original]
  • Takatoshi Wakabayashi, Benesh Joseph, Shuhei Yasumoto, Tomoyoshi Akashi, Toshio Aoki, Kazuo Harada, Satoru Muranaka, Takeshi Bamba, Eiichiro Fukusaki, Yasutomo Takeuchi, Koichi Yoneyama, Toshiya Muranaka, Yukihiro Sugimoto and Atsushi Okazawa (2015) Planteose as a storage carbohydrate required for early stage of germination of Orobanche minor and its metabolism as a possible target for selective control. Journal of Experimental Botany, 66(11): 3085-3097. [Pubmed]

 

[2012]

  • Toda K, Kuroiwa H, Senthil K, Shimada N, Aoki T, Ayabe SI, Shimada S, Sakuta M, Miyazaki Y, Takahashi R. (2012) The soybean F3'H protein is localized to the tonoplast in the seed coat hilum. Planta. 236(1): 79-89. [Pubmed]

 

 

[2011]

  • Seki H, Sawai S, Ohyama K, Mizutani M, Ohnishi T, Sudo H, Fukushima EO, Akashi T, Aoki T, Saito K, Muranaka T. (2011) Triterpene Functional Genomics in Licorice for Identification of CYP72A154 Involved in the Biosynthesis of Glycyrrhizin. Plant Cell,23(11):4112-4123. [PubMed]
  • Sawai S, Uchiyama H, Mizuno S, Aoki T, Akashi T, Ayabe SI, Takahashi T. (2011) Molecular characterization of an oxidosqualene cyclase that yields shionone, a unique tetracyclic triterpene ketone of Aster tataricus. FEBS Letters, 585(7): 1031-1036. [PubMed]
  • Nakagawa T, Kaku H, Shimoda Y, Sugiyama A, Shimamura M, Takanashi K, Yazaki K, Aoki T, Shibuya N, Kouchi H. (2011) From defense to symbiosis: limited alterations in the kinase domain of LysM receptor-like kinases are crucial for evolution of legume-Rhizobium symbiosis. The Plant Journal, 65(2): 169-180. [PubMed]

 

[2010]

  • Yoshida K, Iwasaka R, Shimada N, Ayabe S, Aoki T, Sakuta M. (2010) Transcriptional control of the dihydroflavonol 4-reductase multigene family in Lotus japonicus. Journal of Plant Research, 123(6): 801-805. [PubMed]

 

[2009]

  • 青木俊夫 (2009) マメ科モデル植物のゲノム情報とバイオリソースを利用した天然物生合成遺伝子の同定.ファルマシア,45(9): 881-886 
  • 青木俊夫,明石智義,内山寛,綾部真一 (2009) マメ科特異的5-デオキシ型イソフラボノイド生合成遺伝子の解析からわかってきたこと.植物の生長調節,44(1): 31-42. [Aoki, T., Akashi, T., Uchiyama, H., Ayabe, S. (2009) Functional and structural analysis of the genes involved in the legume-specific 5-deoxyisoflavonoid biosynthesis. 44(1): 31-42.]
  • Akashi, T., Sasaki, K., Aoki, T., Ayabe, S., Yazaki, K. (2009) Molecular cloning and characterization of a cDNA for pterocarpan 4-dimethylallyltransferase catalyzing the key prenylation step in the biosynthesis of glyceollin, a soybean phytoalexin, Plant Physiology, 149 (2): 683-693. [PubMed]

 

[2008]

  • Seki, H., Ohyama, K., Sawai, S., Mizutani, M., Ohnishi, T., Sudo, H., Akashi, T., Aoki, T., Saito, K., Muranaka, T. (2008) Licorice b-amyrin 11-oxidase, a cytochrome P450 with a key role in the biosynthesis of the triterpene sweetener glycyrrhizin. Proceedings of the National Academy of Sciences USA, 105(37):14204-14209. [PubMed]
  • Yamazaki, M., Shibata, M., Nishiyama, Y., Springob, K., Kitayama, M., Shimada, N., Aoki, T., Ayabe, S., Saito, K. (2008) Differential gene expression profiles of red and green forms of Perilla frutescens leading to comprehensive identification of anthocyanin biosynthetic genes. FEBS Journal, 275(13): 3494-3502. [PubMed]

 

[2007]

  • Shimamura, M., Akashi, T., Sakurai, N., Suzuki, H., Saito, K., Shibata, D., Ayabe, S., Aoki, T.(2007) 2-Hydroxyisoflavanone dehydratase is a critical determinant of isoflavone productivity in hairy root cultures of Lotus japonicus. Plant and Cell Physiology, 48 (11): 1652-1657. [PubMed]
  • Shimada, N., Sato, S., Akashi, T., Nakamura, Y., Tabata, S., Ayabe S., Aoki, T. (2007) Genome-wide analyses of the structural gene families involved in the legume-specific 5-deoxyisoflavonoid biosynthesis of Lotus japonicus. DNA Research, 14 (1): 25-36. [PubMed]
  • Takahashi, R., Stephen M. Githiri, S.M., Hatayama, K., Emilyn G. Dubouzet, E.G., Shimada, N., Aoki, T., Ayabe, S., Toda, K., Matsumura, H. (2007) A single-base deletion in soybean flavonol synthase gene is associated with magenta flower color. Plant Molecular Biology, 63(1):125-135.[PubMed]

 

[2006]

  • Shimada, N., Nakatsuka, T., Nishihara, M., Yamamura, S., Ayabe, S., Aoki, T. (2006) Isolation and characterization of a cDNA encoding polyketide reductase in Lotus japonicus, Plant Biotechnology, 23(5): 509-513. [Original]
  • Akashi, T., Koshimizu, S., Aoki, T., Ayabe, S. (2006) Identification of cDNAs encoding pterocarpan reductase involved in isoflavan phytoalexin biosynthesis in Lotus japonicus by EST mining, FEBS Letters, 580(24): 5666-5670. [PubMed]
  • Akashi, T., VanEtten, H.D., Sawada, Y., Wasmann, C.C., Uchiyama, H., Ayabe, S. (2006) Catalytic specificity of pea O-methyltransferases suggests gene duplication for (+)-pisatin biosynthesis, Phytochemistry, 67(23): 2525-2530. [PubMed]
  • Nukui, N., Minamisawa, K., Ayabe, S., Aoki, T. (2006) Expression of 1-aminocyclopropane-1-carboxylic acid deaminase gene requires symbiotic nitrogen-fixing regulator gene nifA2 in Mesorhizobium loti MAFF303099. Applied and Environmental Microbiology, 72(7): 4964-4969.[PubMed]
  • Ayabe, S., Akashi, T. (2006) Cytochrome P450s in flavonoid metabolism. Phytochemistry Reviews, 5(2-3): 271-282. [Abstract]
  • Sawai, S., Akashi, T., Sakurai, N., Suzuki, H., Shibata, D., Ayabe, S., Aoki T. (2006) Plant lanosterol synthase: divergence of the sterol and triterpene biosynthetic pathways in eukaryotes. Plant and Cell Physiology, 47(5): 673-677. [PubMed]
  • Sawai, S., Shindo, T., Sato, S., Kaneko, T., Tabata, S., Ayabe, S., Aoki T. (2006) Functional and structural analysis of genes encoding oxidosqualene cyclases of Lotus japonicus. Plant Science, 170 (2): 247-257. [Abstract]

 

[2005]

  • 綾部真一,貫井憲之,嶋田典基 (2005) マメ科植物と根粒菌のケミカルコミュニケーション.植物の生長調節,40(2): 156-166. [Ayabe, S., Nukui, N., Shimada, N. (2005) Chemical communications between leguminous plants and rhizobia. Regulation of Plant Growth & Development, 40(2): 156-166.]
  • Imaizumi, R., Sato, S., Kameya, N., Nakamura, I., Nakamura, Y., Tabata, S., Ayabe, S., Aoki, T.(2005) Activation tagging approach in a model Legume Lotus japonicus. Journal of Plant Research, 118(6): 391-399. [PubMed]
  • 明石智義,青木俊夫,綾部真一(2005)大豆イソフラボンの生合成に関わる酵素たん白質をコードする遺伝子の発現解析.大豆たん白質研究, 8: 24-29.[Akashi, T., Aoki, T., Ayabe, S.(2005) Expression analysis of genes encoding enzymes involved in the biosynthesis of isoflavones in soybean. Soy Protein Research Japan, 8: 24-29.]
  • Akashi, T., Ishizaki, M., Aoki, T., Ayabe, S. (2005) Isoflavonoid production by adventitious root cultures of Iris germanica (Iridaceae). Plant Biotechnology, 22(3): 207-215.
  • Nakatsukasa-Akune, M., Yamashita, K., Shimoda, Y., Uchiumi, T., Abe, M., Aoki, T., Kamizawa, A., Ayabe, S., Higashi, S., Suzuki, A. (2005) Suppression of root nodule formation by artificial expression of the TrEnodDR1 (coat protein of white clover cryptic virus 1) gene in Lotus japonicus. Molecular Plant-Microbe Interactions, 18(10): 1069-1080. [PubMed]
  • Shimada,N., Sasaki, R., Sato, S., Kaneko, T., Tabata, S., Aoki, T., Ayabe, S. (2005) A comprehensive analysis of six dihydroflavonol 4-reductases encoded by a gene cluster of Lotus japonicus genome. Journal of Experimental Botany, 56(419): 2573-2585. [PubMed]
  • Chen, R., Tsuda, S., Matsui, K., Fukuchi-Mizutani, M., Ochiai, M., Shimizu, S., Sakuradani, E., Aoki, T., Imaizumi, R., Ayabe, S., Tanaka, Y. (2005) Production of g-linolenic acid in Lotus japonicus and Vigna angularis by expression of the D6-fatty-acid desaturase gene isolated from Mortierella alpina. Plant Science, 169(3): 599-605. 
  • Sawada, Y., Ayabe, S. (2005) Multiple mutagenesis of P450 isoflavonoid synthase reveals a key active-site residue. Biochemical and Biophysical Research Communications, 330(3):907-913. [PubMed]
  • Akashi, T., Aoki, T., Ayabe, S. (2005) Molecular and biochemical characterization of 2-hydroxyisoflavanone dehydratase. Involvement of carboxylesterase-like proteins in leguminous isoflavone biosynthesis. Plant Physiology, 137(3): 882-891. [PubMed]

 

[2004]

  • Oguro, S., Akashi, T., Ayabe, S., Noguchi, H., Abe, I. (2004) Probing biosynthesis of plant polyketides with synthetic N-acetylcysteamine thioesters. Biochemical and Biophysical Research Communications, 325(2): 561-567. [PubMed]
  • Kouchi, H., Shimomura, K., Hata, S., Hirota, A., Wu, G.J., Kumagai, H., Tajima, S., Suganuma, N., Suzuki, A., Aoki, T., Hayashi, M., Yokoyama, T., Ohyama, T., Asamizu, E., Kuwata, C., Shibata, D., Tabata, S. (2004) Large-scale analysis of gene expression profiles during early stages of root nodlule formation in a model legume, Lotus japonicus. DNA Research, 11(4): 263-274. [PubMed]
  • Tohyama, S., Eguchi, T., Dhakal, R.P., Akashi, T., Otsuka, M., Kakinuma, K. (2004) Genome-inspired search for new antibiotics. Isolation and structure determination of new 28-membered polyketide macrolactones, halstoctacosanolides A and B, from Streptomyces halstedii HC34. Tetrahedron, 60(18): 3999-4005. 
  • Ohsaki, A., Imai, Y., Naruse, M., Ayabe, S., Komiyama, K., Takashima, J. (2004) Four new triterpenoids from Maytenus ilicifolia. Journal of Natural Products, 67(3): 469-471.

 

[2003]

  • Akashi, T., Sawada, Y., Shimada, N., Sakurai, N., Aoki, T., Ayabe, S. (2003) cDNA cloning and biochemical characterization of S-adenosyl-L-methionine: 2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase, a critical enzyme of the legume isoflavonoid phytoalexin pathway. Plant and Cell Physiology, 44(2):103-112. [PubMed]
  • Shimada,N., Aoki, T., Sato, S., Nakamura, Y., Tabata, S., Ayabe, S. (2003) A cluster of genes encodes the two types of chalcone isomerase involved in the biosynthesis of general flavonoids and legume-specific 5-deoxy(iso)flavonoids in Lotus japonicus. Plant Physiology, 131: 941-951. [PubMed]

 

[2002]

  • Aoki, T., Kamizawa, A., Ayabe, S. (2002) Efficient Agrobacterium-mediated transformation of Lotus japonicus with reliable antibiotic selection. Plant Cell Reports, 21: 238-243. [Abstract]
  • Sawada, Y., Kinoshita, K., Akashi, T., Aoki, T., Ayabe, S. (2002) Key amino acid residues required for aryl migration catalyzed by the cytochrome P450 2-hydroxyisoflavanone synthase. Plant Journal, 31(5): 555-564. [PubMed]
  • Aoki, T., Kitano, H., Kameya, N. and Nakamura, I. (2002) Accelerated shoot overgrowth of rice mutant ao-1 is epistatic to gibberrellin-sensitive and insensitive dwarf mutants. J. Plant Reserch 115: 195-202 [Abstract]
  • 綾部真一,明石智義 (2002) イソフラボノイドの生合成とシトクロムP450:化学結合の切断・転位を伴う酸素化反応による植物機能物質の構築.化学と生物,40(3), 149-151.

 

[2001]

  • Kimura, Y., Aoki, T., Ayabe, S. (2001) Chalcone isomerase isozymes with different substrate specificities towards 6'-hydroxy- and 6'-deoxychalcones in cultured cells of Glycyrrhiza echinata, a leguminous plant producing 5-deoxyflavonoids. Plant and Cell Physiology, 42(10): 1169-1173. [PubMed]

 

[2000]

  • Shimada, N., Akashi, T., Aoki, T., Ayabe, S. (2000) Induction of isoflavonoid pathway in the model legume Lotus japonicus: molecular characterization of enzymes involved in phytoalexin biosynthesis. Plant Science, 160(1): 37-47. [PubMed]
  • Aoki,T., Akashi, T., Ayabe, S. (2000) Flavonoids of leguminous plants: Structure, biological activity, and biosynthesis. Journal of Plant Research, 113 (1112): 475-488. [Abstract]
  • Akashi, T., Sawada, Y. Aoki, T., Ayabe, S. (2000) New scheme of the biosynthesis of formononetin involving 2,7,4'-trihydroxyisoflavanone but not daidzein as the methyl acceptor. Bioscience, Biotechnology, and Biochemistry, 64 (10): 2276-2279. [PubMed]

 

[1999]

  • Akashi, T., Fukuchi-Mizutani, M., Aoki, T., Ueyama, Y., Yonekura-Sakakibara, K., Tanaka, Y., Kusumi. T., Ayabe, S. (1999) Molecular cloning and biochemical characterization of a novel cytochrome P450, flavone synthase II, that catalyzes direct conversion of flavanones to flavones. Plant and Cell Physiology, 40 (11): 1182-1186. [PubMed]
  • Akashi, T., Aoki, T., Ayabe, S. (1999) Cloning and functional expression of a cytochrome P450 cDNA encoding 2-hydroxyisoflavanone synthase involved in biosynthesis of the isoflavonoid skeleton in licorice. Plant Physiology, 121 (3): 821-828. [PubMed]
  • Nakamura, K., Akashi, T., Aoki, T., Kawaguchi, K., Ayabe, S. (1999) Induction of isoflavonoid and retrochalcone branches of flavonoid pathway in cultured Glycyrrhiza echinata cells treated with yeast extract. Bioscience, Biotechnology, and Biochemistry, 63 (9): 1618-1620. [PubMed]

 

[1998]

  • Akashi, T., Aoki, T., Ayabe, S. (1998) CYP81E1, a cytochrome P450 cDNA of licorice (Glycyrrhiza echinata L.), encodes isoflavone 2'-hydroxylase. Biochemical and Biophysical Research Commnications, 251(1): 67-70. [PubMed]
  • Akashi, T., Aoki, T., Ayabe, S. (1998) Identification of a cytochrome P450 cDNA encoding (2S)-flavanone 2-hydroxylase of licorice (Glycyrrhiza echinata L.) which represents licodione synthase and flavone synthase II. FEBS Letters, 431(2): 287-290. [PubMed]
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